Substrate requirements and kinetic analysis of the cortisol effects on uridine uptake and incorporation by mouse lymphoma P1798 cells in vitro.
نویسندگان
چکیده
Cortisol markedly suppressed in vitro utilization of uridine-3 H by corticoid-sensitive mouse lymphoma P1798 cells. Corticoid-resistant cells were not affected. Inhibition did not depend on reduced uptake of exogenous carbohydrate precursors since similar effects were obtained in glucose-free, pyruvate-free medium. Omission of glucose and pyruvate per se did not influence uridine-3H uptake and incorporation by
منابع مشابه
Substrate Requirements and Kinetic Analysis of the Cortisol Effects on Uridine Uptake and Incorporation by Mouse Lymphoma P1798 Cells in Vitro1
Cortisol markedly suppressed in vitro utilization of uridine-3 H by corticoid-sensitive mouse lymphoma P1798 cells. Corticoid-resistant cells were not affected. Inhibition did not depend on reduced uptake of exogenous carbohydrate precursors since similar effects were obtained in glucose-free, pyruvate-free medium. Omission of glucose and pyruvate per se did not influence uridine-3H uptake and ...
متن کاملEffects of cortisol on DNA metabolism in the sensitive and resistant lines of mouse lymphoma P1798.
Administration of cortisol to mice bearing the cortisol sensitive or -resistant lines of lymphosarcoma P1 798 resulted in a marked inhibition of incorporation of labeled deoxy nucleoside precursors into tumor DNA only in the sensitive tumor. This effect is one of the earliest biochemical changes see n in lymphosarcoma P1798 following glucocorticoid treatment. It is dependent on both time and do...
متن کاملInhibitory effect of cortisol in vitro on 2-deoxyglucose uptake and RNA and protein metabolism in lymphosarcoma P1798.
nonmetabolizable amino acid was significantly inhibited (20%) only in cells exposed to cortisol 2 hr. When glucose was omitted from the incubation medium, the incorporation of radioactive precursors into RNA and protein was inhibited by 80 to 85%; under the same conditions, inhibition produced by cortisol was only 49 and 63%, respectively, of that observed when glucose was present in the medium...
متن کاملSequential irreversible, actinomycin D-sensitive, and cycloheximide-sensitive steps prior to cortisol inhibition of uridine utilization by P1798 tumor lymphocytes.
Events preceeding the cortisol inhibition of uridine utilization by corticoid-sensitive P1798 lymphocytes have been investigated. When tumor cells were incubated with 1 muM cortisol for 15 min and then washed free of steroid and reincubated in the absence of hormone, the expected decrease of uridine uptake failed to appear 1.5 hr later. In contrast, the removal of cortisol after 30 or 60 min di...
متن کاملGlucocorticoid action on rat thymus cells. II. Interrelationships between ribonucleic acid and protein metabolism and between cortisol and substrate effects on these metabolic parameters in vitro.
In rat thymus cells incubated with carbohydrate substrate the steady state rate of incorporation of uridine into RNA generally parallels the rate of incorporation of amino acids into protein. In both instances glucose is required for continued maximal incorporation rates, a requirement that is only partially satisfied by other readily metabolized substrates such as pyruvate, lactate, or /3-hydr...
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ورودعنوان ژورنال:
- Cancer research
دوره 34 9 شماره
صفحات -
تاریخ انتشار 1974